منابع مشابه
Group B arbovirus structural and nonstructural antigens. 3. Serological specificity of solubilized intracellular viral proteins.
Solubilized nonstructural antigen III from Saint Louis encephalitis (SLE)-Japanese B encephalitis (JBE)-West Nile (WN) and dengue-2 arbovirus-infected pig kidney cells was purified by employing Brij-58 solubilization, organic solvent extraction, and column chromatography. Diethylaminoethyl-cellulose column peak C eluate contained only intracellular viral envelope protein designated antigen I. A...
متن کاملInhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B.
A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-speci...
متن کاملAntigenic components of group A arbovirus virions.
Three group A arboviruses, Sindbis (SIN), western (WEE) and eastern equine encephalitis (EEE), were selectively degraded with a nonionic detergent to yield a core particle and a soluble envelope component. Antigenic analysis by using radioimmune precipitation techniques revealed marked antigenic similarity among the core particles of the three viruses. The soluble envelope component exhibited a...
متن کاملGroup B arbovirus structural and nonstructural antigens. II. Purification of Saint Louis encephalitis virus intracellular antigens.
Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose column contained the intracellular majo...
متن کاملArbovirus infection increases with group size.
Buggy Creek (BCR) virus is an arthropod-borne alphavirus that is naturally transmitted to its vertebrate host the cliff swallow (Petrochelidon pyrrhonota) by an invertebrate vector, namely the cimicid swallow bug (Oeciacus vicarius). We examined how the prevalence of the virus varied with the group size of both its vector and host. The study was conducted in southwestern Nebraska where cliff sw...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1969
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.4.5.688-693.1969